Figure 1. Inhibition of GRPR decreased GRP secretion in human neuroblastoma cells. (A) BE(2)-C cells stably-transfected with either shCON or shGRPR were plated in serum-free media for 48 h and analyzed for GRP secretion by ELISA. (B) HUVECs were plated on 24-well plates coated with Matrigel and incubated with BE(2)-C cell culture media from either shCON or shGRPR. After 6 h, cells were fixed and stained with H&E. Tubules were examined by microscopy and average number of tubules was counted from three separate fields of view. (C) BE(2)-C and BE(2)-M17 cells were plated in serum-free media and treated with DMSO or RC-3095 (1 μM) for 48 h. ELISA was used to analyze GRP secretion. (D) HUVECs were plated on 24-well plates coated with Matrigel and incubated with cell culture media from BE(2)-C or BE(2)-M17 cells treated with DMSO or RC-3095. Similar to (B), tubules were examined by microscopy. Values shown are mean ± SEM of three separate experiments (*P < 0.05 vs. shCON or without RC-3095).