Figure 4.
Noradrenergic innervation of thalamic trigeminovascular neurons. (A) Left: Immunopositive Dopamine β-Hydroxylase axons (green) surrounding a thalamic dura-sensitive neuron (red) labeled with TMR–dextran. Nuclear counterstaining was performed with DAPI (blue). Arrowheads indicate close apposition of DBH positive axons and the cell body and dendrites of the labeled neuron. Upper right: Location of the dura-sensitive neuron (red star) shown at left. Number in red indicates distance from bregma (mm). Lower right: Fluorescent image showing DBH labeling of cell bodies in the LC of the brainstem. Scale bars = 100 mm. (B) Close apposition between DBH immunopositive axons and thalamic trigeminovascular neurons. The three views in the x-y, y-z and x-z planes provide evidence that DBH immunopositive fibers (green) may contact cell bodies, proximal and distal dendrites of trigeminovascular neurons in Po (red). Arrowheads indicate probable contact point on each view. Note that some green-labeled axons and red-labeled soma or dendrites are in the same focal plane (yellow). Scale bar = 50 μm. Adapted from Noseda et al., 201427