Abstract
Between days 10 and 21 after inoculation of Lupinus angustifolius seedlings with Rhizobium NZP 2257, the average nodule fresh weight increased 3-fold and the number of bacteroids per nodule increased more than 10-fold.
The viability of Rhizobium bacteroids, as judged by their ability to form colonies on yeast-extract agar, declined from about 10% on days 10 and 11 after inoculation to about 0.3% on days 14 to 25. Bacteroid viability was highly sensitive to osmolarity.
At optimal pH and K and Mg ion concentrations, the incorporation of 14C-glycine into isolated bacteroids was also very sensitive to osmolarity, and fell in parallel with bacteroid viability during nodule development.
We suggest that at least two processes contribute to bacteroid nonviability: a reversible change in the cell wall structure occurring between days 10 and 14 after inoculation, and a subsequent irreversible change.
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Selected References
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