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. 1977 Jun;59(6):1064–1066. doi: 10.1104/pp.59.6.1064

Accumulation of Free Ricinoleic Acid in Germinating Castor Bean Endosperm 1

Robert P Donaldson a,2
PMCID: PMC542507  PMID: 16659994

Abstract

Lipids from the endosperm of germinating castor bean (Ricinus communis var. Hale) were separated by thin layer chromatography and quantitated by gas chromatography. During the later stages of lipid breakdown (4-6 days germination at 30 C), several lipid classes were found in addition to the storage triglycerides, which are triricinoleins for the most part. One was identified as free ricinoleic acid, the proportion of which increased as germination progressed. After 6 days germination, ricinoleic acid comprised more than 30% of the total lipid. The appearance of this fatty acid implies that lipase activity (lipolysis) is not strictly coordinated with β oxidation in this tissue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEEVERS H. Metabolic production of sucrose from fat. Nature. 1961 Jul 29;191:433–436. doi: 10.1038/191433a0. [DOI] [PubMed] [Google Scholar]
  2. CANVIN D. T., BEEVERS H. Sucrose synthesis from acetate in the germinating castor bean: kinetics and pathway. J Biol Chem. 1961 Apr;236:988–995. [PubMed] [Google Scholar]
  3. Donaldson R. P., Beevers H. Lipid composition of organelles from germinating castor bean endosperm. Plant Physiol. 1977 Feb;59(2):259–263. doi: 10.1104/pp.59.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Donaldson R. P. Membrane lipid metabolism in germinating castor bean endosperm. Plant Physiol. 1976 Apr;57(4):510–515. doi: 10.1104/pp.57.4.510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Donaldson R. P., Tolbert N. E., Schnarrenberger C. A comparison of microbody membranes with microsomes and mitochondria from plant and animal tissue. Arch Biochem Biophys. 1972 Sep;152(1):199–215. doi: 10.1016/0003-9861(72)90208-1. [DOI] [PubMed] [Google Scholar]
  6. Gerhardt B. P., Beevers H. Developmental studies on glyoxysomes in Ricinus endosperm. J Cell Biol. 1970 Jan;44(1):94–102. doi: 10.1083/jcb.44.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Marriott K. M., Northcote D. H. The breakdown of lipid reserves in the endosperm of germinating castor beans. Biochem J. 1975 Apr;148(1):139–144. doi: 10.1042/bj1480139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Marriott K. M., Northcote D. H. The induction of enzyme activity in the endosperm of germinating castor-bean seeds. Biochem J. 1975 Oct;152(1):65–70. doi: 10.1042/bj1520065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Muto S., Beevers H. Lipase Activities in Castor Bean Endosperm during Germination. Plant Physiol. 1974 Jul;54(1):23–28. doi: 10.1104/pp.54.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. ORY R. L., ST ANGELO A. J., ALTSCHUL A. M. Castor bean lipase: action on its endogenous substrate. J Lipid Res. 1960 Apr;1:208–213. [PubMed] [Google Scholar]
  11. Ory R. L., Yatsu L. Y., Kircher H. W. Association of lipase activity with the spherosomes of Ricinus communis. Arch Biochem Biophys. 1968 Feb;123(2):255–264. doi: 10.1016/0003-9861(68)90132-x. [DOI] [PubMed] [Google Scholar]
  12. Vigil E. L. Cytochemical and developmental changes in microbodies (glyoxysomes) and related organelles of castor bean endosperm. J Cell Biol. 1970 Sep;46(3):435–454. doi: 10.1083/jcb.46.3.435. [DOI] [PMC free article] [PubMed] [Google Scholar]

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