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. 1977 Jun;59(6):1082–1084. doi: 10.1104/pp.59.6.1082

Isolation of Uridine 5′-Pyrophosphate Glucuronic Acid Pyrophosphorylase and Its Assay Using 32P-Pyrophosphate 1

David B Dickinson a, David Hyman a,2, Jorge W Gonzales a,3
PMCID: PMC542511  PMID: 16659998

Abstract

A procedure was devised to detect and assay uridine 5′-pyrophosphate (UDP)-glucuronic acid pyrophosphorylase in plant extracts. Substrates are UDP-glucuronic acid and 32P-pyrophosphate, and the 32P-uridine 5′-triphosphate produced is selectively adsorbed to charcoal. The charcoal adsorption procedure is a modification of that used to determine 32P-adenosine 5′-triphosphate produced by adenosine 5′-pyrophosphate glucose pyrophosphorylase, and the modification greatly improves the retention of uridine 5′-triphosphate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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