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. 1977 Jun;59(6):1178–1181. doi: 10.1104/pp.59.6.1178

Strong Binding of Cytochrome c on the Envelope of Spinach Chloroplasts

Michel Neuburger 1, Jacques Joyard 1, Roland Douce 1
PMCID: PMC542530  PMID: 16660017

Abstract

Yeast cationic ferricytochrome c was able to bind to the spinach (Spinacia oleracea) chloroplast envelope with a low affinity (Kd = 1.1 μm). The total amount of low affinity binding sites was of the order of 50 nmol cytochrome c mg−1 protein. We gave the evidence that binding of ferricytochrome c to the envelope was electrostatic and that the envelope membranes were strongly negatively charged. Addition of yeast ferricytochrome c to a preparation of intact washed chloroplasts (class I) induced a strong agglutination of chloroplasts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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