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. 1977 Jul;60(1):26–29. doi: 10.1104/pp.60.1.26

Assay for Helminthosporium maydis Toxin-binding Activity in Plants 1

Carrie R Ireland a,2, Gary A Strobel a,3
PMCID: PMC542539  PMID: 16660035

Abstract

A relatively rapid and sensitive assay is described for assessing the binding of Helminthosporium maydis Race T14 C-toxins I and II to plant components. The technique is a modification of the one of Haddad and Birge (J. Biol. Chem. 250: 299-303, 1975), and utilizes dextran-coated charcoal as an adsorbent for the unreacted toxin and employs a Millipore filter to isolate the protein-toxin complex.

Extracts from susceptible corn line W64A Tms possess a protein primarily localized in the cytosol which is relatively heat-insensitive, ficin- and papain-sensitive, and binds toxins I and II at half-saturation in the order of 0.1 mm. The toxin-binding activities of the extracts of various corn lines and other species are not correlated to resistance or susceptibility to H. maydis Race T, nor to sensitivity to the toxins. These findings are discussed relative to the function of the binding protein and cellular sensitivity to the toxins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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