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. 1977 Jul;60(1):51–53. doi: 10.1104/pp.60.1.51

Reversal of 3-(3,4-Dichlorophenyl)-1,1-Dimethylurea Inhibition of Carbon Dioxide Fixation in Spinach Chloroplasts and Protoplasts by Dicarboxylic Acids 1

C K M Rathnam a, Barbara A Zilinskas a
PMCID: PMC542545  PMID: 16660042

Abstract

3-(3,4-Dichlorophenyl)-1,1-dimethylurea (DCMU) inhibition of 14CO2 fixation in isolated intact spinach (Spinacia oleracea L.) chloroplasts was reversed (by about 34%) by l-malate but not by oxaloacetate (OAA). However, OAA reversed the DCMU inhibition in spinach protoplasts indicating an extrachloroplastic enzyme requirement. Extrachloroplastic OAA reduction was coupled with external dihydroxyacetone phosphate (DHAP) oxidation, and the malate formed from such coupling might then enter the chloroplasts. Evidence was presented using ruptured protoplasts that the export of recently formed 3-phosphoglyceric acid (PGA) out of chloroplasts in exchange for external DHAP was reversed by excess OAA. The PGA/DHAP shuttle across the chloroplast envelope was found to be regulated by the external concentrations of DHAP and OAA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Heber U., Kirk M. R. Flexibility of coupling and stoichiometry of ATP formation in intact chloroplasts. Biochim Biophys Acta. 1975 Jan 31;376(1):136–150. doi: 10.1016/0005-2728(75)90212-1. [DOI] [PubMed] [Google Scholar]
  2. Heber U., Santarius K. A. Direct and indirect transfer of ATP and ADP across the chloroplast envelope. Z Naturforsch B. 1970 Jul;25(7):718–728. doi: 10.1515/znb-1970-0714. [DOI] [PubMed] [Google Scholar]
  3. Heldt H. W., Rapley L. Specific transport of inorganic phosphate, 3-phosphoglycerate and dihydroxyacetonephosphate, and of dicarboxylates across the inner membrane of spinach chloroplasts. FEBS Lett. 1970 Oct 5;10(3):143–148. doi: 10.1016/0014-5793(70)80438-0. [DOI] [PubMed] [Google Scholar]
  4. Kaiser W., Urbach W. Rates and properties of endogenous cyclic photophosphorylation of isolated intact chloroplasts measured by CO2 fixation in the presence of dihydroxyacetone phosphate. Biochim Biophys Acta. 1976 Jan 15;423(1):91–102. doi: 10.1016/0005-2728(76)90103-1. [DOI] [PubMed] [Google Scholar]
  5. Kanai R., Edwards G. E. Purification of enzymatically isolated mesophyll protoplasts from c(3), c(4), and crassulacean Acid metabolism plants using an aqueous dextran-polyethylene glycol two-phase system. Plant Physiol. 1973 Nov;52(5):484–490. doi: 10.1104/pp.52.5.484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lendzian K., Bassham J. A. Regulation of glucose-6-phosphate dehydrogenase in spinach chloroplasts by ribulose 1,5-diphosphate and NADPH/NADP+ ratios. Biochim Biophys Acta. 1975 Aug 11;396(2):260–275. doi: 10.1016/0005-2728(75)90040-7. [DOI] [PubMed] [Google Scholar]
  7. Nishimura M., Graham D., Akazawa T. Isolation of intact chloroplasts and other cell organelles from spinach leaf protoplasts. Plant Physiol. 1976 Sep;58(3):309–314. doi: 10.1104/pp.58.3.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Santarius K. A., Stocking C. R. Intracellular localization of enzymes in leaves and chloroplast membrane permeability to compounds involved in amino acid syntheses. Z Naturforsch B. 1969 Sep;24(9):1170–1179. doi: 10.1515/znb-1969-0915. [DOI] [PubMed] [Google Scholar]
  9. Werdan K., Heldt H. W., Milovancev M. The role of pH in the regulation of carbon fixation in the chloroplast stroma. Studies on CO2 fixation in the light and dark. Biochim Biophys Acta. 1975 Aug 11;396(2):276–292. doi: 10.1016/0005-2728(75)90041-9. [DOI] [PubMed] [Google Scholar]
  10. Wintermans J. F., de Mots A. Spectrophotometric characteristics of chlorophylls a and b and their pheophytins in ethanol. Biochim Biophys Acta. 1965 Nov 29;109(2):448–453. doi: 10.1016/0926-6585(65)90170-6. [DOI] [PubMed] [Google Scholar]

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