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. 1977 Jul;60(1):89–94. doi: 10.1104/pp.60.1.89

Evolutionary Conservation of Chloroplast Genes Coding for the Large Subunits of Fraction 1 Protein

Shain-Dow Kung 1, Ching-Ing Lee 1, Denise D Wood 2, Mario A Moscarello 2
PMCID: PMC542554  PMID: 16660051

Abstract

Crystalline fraction 1 protein, obtained from four species of Nicotiana, have identical polypeptide compositions and isoelectric points. However, the tryptic peptide map of the large subunit of this protein from N. knightiana and N. paniculata differs from that of N. tomentosa and N. tomentosiformis. Since the large subunits of fraction 1 protein are coded by chloroplast DNA, the difference in their primary structure reflects the structural changes of the chloroplast genes containing the coding information. This indicates that the rate of mutation of chloroplast DNA seems to be higher than predicated from the analysis of isoelectric points of this protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker T. S., Eisenberg D., Eiserling F. A., Weissman L. The structure of form I crystals of D-ribulose-1,5-diphosphate carboxylase. J Mol Biol. 1975 Feb 5;91(4):391–399. doi: 10.1016/0022-2836(75)90267-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bottomley W., Higgins T. J., Whitfeld P. R. Differential recognition of chloroplast and cytoplasmic messenger RNA by 70S and 80S ribosomal systems. FEBS Lett. 1976 Mar 15;63(1):120–124. doi: 10.1016/0014-5793(76)80207-4. [DOI] [PubMed] [Google Scholar]
  3. Chan P. H., Sakano K., Singh S., Wildman S. G. Crystalline fraction I protein: preparation in large yield. Science. 1972 Jun 9;176(4039):1145–1146. doi: 10.1126/science.176.4039.1145. [DOI] [PubMed] [Google Scholar]
  4. Chan P. H., Wildman S. G. Chloroplast DNA codes for the primary structure of the large subunit of fraction I protein. Biochim Biophys Acta. 1972 Sep 14;277(3):677–680. doi: 10.1016/0005-2787(72)90115-3. [DOI] [PubMed] [Google Scholar]
  5. Kawashima N., Kwok S. Y., Wildman S. G. Studies on fraction-I protein. 3. Comparison of the primary structure of the large and small subunits obtained from five species of Nicotiana. Biochim Biophys Acta. 1971 Jun 29;236(3):578–586. doi: 10.1016/0005-2795(71)90242-x. [DOI] [PubMed] [Google Scholar]
  6. Kung S. D., Sakano K., Wildman S. G. Multiple peptide composition of the large and small subunits of Nicotiana tabacum fraction I protein ascertained by fingerprinting and electrofocusing. Biochim Biophys Acta. 1974 Sep 13;365(1):138–147. doi: 10.1016/0005-2795(74)90258-x. [DOI] [PubMed] [Google Scholar]
  7. Kung S. D., Thornber J. P., Wildman S. G. Nuclear DNA codes for the photosystem II chlorophyll-protein of chloroplast membranes. FEBS Lett. 1972 Aug 1;24(2):185–188. doi: 10.1016/0014-5793(72)80763-4. [DOI] [PubMed] [Google Scholar]
  8. Kung S. Tobacco fraction 1 protein: a unique genetic marker. Science. 1976 Feb 6;191(4226):429–434. doi: 10.1126/science.1108201. [DOI] [PubMed] [Google Scholar]
  9. McFadden B. A., Tabita F. R. D-ribulose-1, 5-diphosphate carboxylase and the evolution of autotrophy. Biosystems. 1974 Oct;6(2):93–112. doi: 10.1016/0303-2647(74)90002-1. [DOI] [PubMed] [Google Scholar]
  10. Nishimura M., Akazawa T. Further proof for the catalytic role of the larger subunit in the spinach leaf ribulose-1,5-diphosphate carboxylase. Biochem Biophys Res Commun. 1973 Oct 1;54(3):842–848. doi: 10.1016/0006-291x(73)90770-5. [DOI] [PubMed] [Google Scholar]
  11. Rutner A. C., Lane M. D. Nonidentical subunits of ribulose diphosphate carboxylase. Biochem Biophys Res Commun. 1967 Aug 23;28(4):531–537. doi: 10.1016/0006-291x(67)90346-4. [DOI] [PubMed] [Google Scholar]
  12. Singh S., Wildman S. G. Chloroplast DNA codes for the ribulose diphosphate carboxylase catalytic site on fraction I proteins of Nicotiana species. Mol Gen Genet. 1973 Aug 17;124(3):187–196. doi: 10.1007/BF00293090. [DOI] [PubMed] [Google Scholar]
  13. Thomas J. R., Tewari K. K. Conservation of 70S ribosomal RNA genes in the chloroplast DNAs of higher plants. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3147–3151. doi: 10.1073/pnas.71.8.3147. [DOI] [PMC free article] [PubMed] [Google Scholar]

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