Abstract
A rapid, simple method for nuclei isolation and purification from soybean (Glycine max L. Merr.) protoplasts is described. The isolated nuclei exhibited active amino acid incorporation and RNA synthesis, but DNA synthesis was not detectable. Analysis by CsCl density gradient centrifugation showed that DNA isolated from nuclei had a single band, while DNA isolated from protoplasts consisted of three bands comprised of nuclear DNA, mitochondrial DNA, and chloroplast DNA.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bernard O., Brent T. P. DNA replication in isolated HeLa cell nuclei. Biochem Biophys Res Commun. 1973 Aug 21;53(4):1213–1219. doi: 10.1016/0006-291x(73)90594-9. [DOI] [PubMed] [Google Scholar]
- Chen Y. M., Lin C. Y., Chang H., Guilfoyle T. J., Key J. L. Isolation and Properties of Nuclei from Control and Auxin-treated Soybean Hypocotyl. Plant Physiol. 1975 Jul;56(1):78–82. doi: 10.1104/pp.56.1.78. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gamborg O. L. The effects of amino acids and ammonium on the growth of plant cells in suspension culture. Plant Physiol. 1970 Apr;45(4):372–375. doi: 10.1104/pp.45.4.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ohyama K., Gamborg O. L., Miller R. A. Isolation and Properties of Deoxyribonucleic Acid from Protoplasts of Cell Suspension Cultures of Ammi visnaga and Carrot (Daucus carota). Plant Physiol. 1972 Sep;50(3):319–321. doi: 10.1104/pp.50.3.319. [DOI] [PMC free article] [PubMed] [Google Scholar]