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. 1977 Aug;60(2):197–202. doi: 10.1104/pp.60.2.197

Kinetin Incorporated into Tobacco Callus Ribosomal RNA and Transfer RNA Preparations 1

Norimoto Murai a, Barbara J Taller a, Donald J Armstrong a,2, Folke Skoog a, Melvin A Micke b, Heinrich K Schnoes b
PMCID: PMC542578  PMID: 16660058

Abstract

Kinetin, N6-furfuryladenine, was incorporated into tobacco (nicotiana tabacum L., var. Wis. No. 38) callus RNA isolated from rapidly growing tissue cultured in the presence of N6-furfuryladenine-8-14C or unlabeled kinetin. Approximately 0.7% of the radioactivity in the labeled kinetin added to the medium was recovered as N6-furfuryladenosine (fr6A) in the rRNA and tRNA preparations from the tobacco callus. The rRNA contained over 90% of these fr6 A moieties. The extent of kinetin incorporation was four times greater than that observed for N6-benzyladenine. The radiochemical purity of the recovered fr6 A was confirmed by three successive chromatographic purifications on Sephadex columns (LH-20 eluted with 35% ethanol, G-10 eluted with 20% ethanol, and LH-20 eluted with water). A cytokinin-active ribonucleoside with elution volumes corresponding to fr6 A was isolated from the tobacco callus rRNA preparation. This compound was analyzed by gas-liquid chromatography and rigorously characterized as N6-furfuryladenosine by gas-liquid chromatography-mass spectrometry of the trimethylsilyl derivative.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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