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. 1977 Oct;60(4):478–481. doi: 10.1104/pp.60.4.478

Isolation and Purification of an α-Mannosidase from Coleoptiles of Avena sativa1

L Carl Greve a,2, Lawrence Ordin a,3
PMCID: PMC542645  PMID: 16660119

Abstract

An α-mannosidase has been purified from the coleoptiles of Avena sativa L. var. Segrehavre. The enzyme, which is tightly associated with the cell wall, was solubilized with 3 m LiCl. The purification involves precipitation with (NH4)2SO4, gel filtration, ion exchange chromatography, and isoelectric focusing. The enzyme appears homogeneous when chromatographed on disc gels and on isoelectric focusing gels. The enzyme runs as a single protein of constant specific activity when chromatographed on Sephadex G-200. The estimated molecular weight of the enzyme is 630,000. The enzyme appears to have no metal ion cofactor requirement and is insensitive to p-chloromercuribenzoate. The pH optimum for the enzyme with p-nitrophenyl-α-d-mannoside as the substrate is 4.5 and the Km is 3.2 mm. The enzyme may have some carbohydrate associated with it as indicated by a positive periodate-Schiff reaction on disc gels.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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