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. 1977 Oct;60(4):492–495. doi: 10.1104/pp.60.4.492

Influence of pH upon the Warburg Effect in Isolated Intact Spinach Chloroplasts

II. Interdependency of Glycolate Synthesis upon pH and Calvin Cycle Intermediate Concentration in the Absence of Carbon Dioxide Photoassimilation 1

Yoke Wah Kow a, J Michael Robinson a,2, Martin Gibbs a
PMCID: PMC542648  PMID: 16660122

Abstract

The light-dependent synthesis of glycolate derived from fructose 1,6-diphosphate, ribose 5-phosphate, or glycerate 3-phosphate was studied in the intact spinach (Spinacia oleracea) chloroplasts in the absence of CO2. Glycolate yield increased with an elevation of O2, pH, and the concentration of the phosphorylated compound supplied. No pH optimum was observed as the pH was increased from 7.4 to 8.5. The average maximal rate of glycolate synthesis was 50 μmoles per milligram chlorophyll per hour while the highest rate observed was 92 with 2.5 mm fructose 1,6-diphosphate in 100% O2. The highest yields of glycolate synthesized from fructose 1,6-diphosphate, ribose 5-phosphate, or glycerate 3-phosphate were 0.14, 0.24, and 0.30, respectively, on a molar basis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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