Abstract
Fruits of tomato (Lycopersicon esculentum Mill.) cv. Rutgers and of a nearly isogenic stock containing the ripening inhibitor gene rin harvested at green (66% mature) and ripe (107% mature) stages were studied for the subcellular distribution of isoenzymes using isoelectric focusing. The enzymes studied were peroxidases, esterases, phosphatases, phosphorylase, malate dehydrogenases, and IAA oxidases. During ripening of normal fruit the activities in the supernatant fraction of all of these enzymes, except malate dehydrogenase, decreased. In the particulate fractions some enzymes decreased while others increased in activity. The rin gene inhibited only some of the changes which occurred during ripening of normal fruit. It is postulated that changes in the degree to which enzymes are bound to membranes comprise one of the mechanisms by which the activities of enzymes are controlled in tomato pericarp, and that these membranes remain intact during ripening.
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Selected References
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- Herner R. C., Sink K. C. Ethylene Production and Respiratory Behavior of the rin Tomato Mutant. Plant Physiol. 1973 Jul;52(1):38–42. doi: 10.1104/pp.52.1.38. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mizrahi Y., Dostal H. C., McGlasson W. B., Cherry J. H. Transplantation Studies with Immature Fruit of Normal, and rin and nor Mutant Tomatoes. Plant Physiol. 1975 Jun;55(6):1120–1122. doi: 10.1104/pp.55.6.1120. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Srere P. A., Mosbach K. Metabolic compartmentation: symbiotic, organellar, multienzymic, and microenvironmental. Annu Rev Microbiol. 1974;28(0):61–83. doi: 10.1146/annurev.mi.28.100174.000425. [DOI] [PubMed] [Google Scholar]