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. 1977 Oct;60(4):524–527. doi: 10.1104/pp.60.4.524

Appearance of an Alternate Pathway Cyanide-resistant during Germination of Seeds of Cicer arietinum

Placido De La Fuente Burguillo 1, Gregorio Nicolás 1
PMCID: PMC542656  PMID: 16660130

Abstract

The combined action of the inhibitors antimycin A and cyanide with benzohydroxamic acid indicates the presence of a cyanide-resistant pathway of respiration in chick pea (Cicer arietinum L.) seeds. The appearance of this pathway takes place during germination. During the first 12 hours of germination, the respiration is predominantly cyanide-sensitive, showing after this time a shift to an “alternate” respiration which is sensitive to benzohydroxamic acid, reaching the maximal cyanide resistance between 72 and 96 hours of germination. The appearance of the alternate pathway is initiated by high O2 concentrations and depends on cytoplasmic protein synthesis, since its appearance is inhibited by cycloheximide but not by chloramphenicol. Actinomycin D has no effect on the appearance of the alternate pathway. Our results indicate, in agreement with other authors, that the branching point is located between the flavoproteins and cytochromes b, probably at the level of ubiquinone, but the possibility of more than one branching point of the electron flow is also considered.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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