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. 1977 Oct;60(4):625–628. doi: 10.1104/pp.60.4.625

Isolation and Oxidative Properties of Intact Mitochondria Isolated from Spinach Leaves 1

Roland Douce a, Antony L Moore a,2, Michel Neuburger a
PMCID: PMC542677  PMID: 16660151

Abstract

A procedure was described for preparing intact mitochondria from spinach (Spinacia oleracea L.) leaves. These mitochondria oxidized succinate, malate, pyruvate, α-ketoglutarate, and NADH with good respiratory control and ADP/O ratios comparable to those observed with mitochondria from other plant tissues. Glycine was oxidized by the preparations. This oxidation linked to the mitochondrial electron transport chain, was coupled to three phosphorylation sites and was sensitive to electron transport and phosphorylation inhibitors.

Cyanide completely inhibited the oxidation of NADH. The oxidation of succinate, malate, and glycine was only partially inhibited.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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