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. 1977 Nov;60(5):675–679. doi: 10.1104/pp.60.5.675

Mitochondria of Isolated Plant Cells (Acer pseudoplatanus L.)

II. Copper Deficiency Effects on Cytochrome C Oxidase and Oxygen Uptake

Richard Bligny a, Roland Douce a
PMCID: PMC542692  PMID: 16660162

Abstract

The effects of copper deficiency on cell culture growth, cell respiration, mitochondrial oxidative properties, and electron transport chain have been studied with suspension-cultured sycamore cells (Acer pseudoplatanus L.). Within the range of the copper concentration studied (0.1-25 μg/1 of culture medium), the mean rate of cell division is independent of copper concentration. An initial copper concentration lower than 2 μg/1 limited the maximum density of population reached at the stationary phase of growth.

On a protein basis, the uncoupled O2 uptake rates were about the same for normal and copper-deficient cells. In contrast, the half-maximal inhibition of O2 uptake rate was obtained at greater KCN concentration in the normal cells (20 μM) compared to copper-deficient cells (2 μM). Similar results were obtained with the normal and copper-deficient sycamore cell mitochondria.

In the copper-deficient mitochondria, the concentration of the cytochrome aa3 was less than 0.02 nmol/mg mitochondrial protein or 1/20 of the normal rate. The b- and c-type cytochrome content was invariant with copper depletion. It appeared that cytochrome aa3 is present in large excess in normal cells. This work also indicated that cytochrome c is a very mobile molecule.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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