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. 1977 Dec;60(6):923–927. doi: 10.1104/pp.60.6.923

Cytochemical Localization of K+-stimulated Adenosine Triphosphatase Activity in Xylem Parenchyma Cells of Barley Roots 1

Erika Winter-Sluiter a,2, André Läuchli a,2, Detlef Kramer a
PMCID: PMC542746  PMID: 16660213

Abstract

ATPase activity in xylem parenchyma cells of barley (Hordeum vulgare L.) roots was demonstrated cytochemically with a lead precipitation reaction. The methodical parameters of this cytochemical test were optimized for distinction between ATPase-specific and nonspecific precipitates. Optimum conditions were prefixation in 1% glutaraldehyde for 1 hour and incubation for 2 hours in a medium containing 2 mm each of ATP, Ca2+, and Pb2+ at pH 7 and 25 C. Problems of cytochemical localizations are discussed.

ATPase activity occurred mainly at the plasmalemma, the endoplasmic reticulum nuclear envelope, and outer mitochondrial membranes of xylem parenchyma cells. The tonoplast of these cells showed only little ATPase activity. High K+ concentrations stimulated ATPase activity, particularly at the plasmalemma. Diethylstilbestrol prevented the formation of ATPase-specific precipitates. The cytochemical demonstration of a K+-stimulated ATPase at the plasmalemma of xylem parenchyma cells is discussed in relation to the possible role of this membrane in ion transport to the vessels.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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