Abstract
Several lines of evidence implicate ammonium as an important factor in the growth and development of cotton (Gossypium hirsutum L.) ovules cultured in vitro. For example, ovules cultured at 28 C require indoleacetic acid (IAA) and either ammonium or gibberellic acid (GA3) in the medium for fiber development, whereas ovules cultured at 34 C require only IAA. Because of this effect of ammonium supply, it seemed possible that hormones or increased temperature were also promoting the availability of reduced nitrogen by induction of increased nitrate reductase activity in the ovules. This possibility was tested.
In vivo, where ovules received mostly reduced nitrogen and very little nitrate, they did not display appreciable nitrate reductase activity even when nitrate was forced into the ovary wall by transpiration. After initiation of culture, nitrate became freely available to ovules and their nitrate reductase activity increased rapidly. Treatment with ammonium, GA3, IAA, or increased temperature had no effect upon this induction. It is concluded that ammonium, hormone, and temperature effects on fiber development are independent of the availability of reduced nitrogen as a general substrate for growth.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
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