Abstract
Changes in neutral sugar, uronic acid, and protein content of tomato (Lycopersicon esculentum Mill) cell walls during ripening were characterized. The only components to decline in amount were galactose, arabinose, and galacturonic acid. Isolated cell walls of ripening fruit contained a water-soluble polyuronide, possibly a product of in vivo polygalacturonase action. This polyuronide and the one obtained by incubating walls from mature green fruit with tomato polygalacturonase contained relatively much less neutral sugar than did intact cell walls. The ripening-related decline in galactose and arabinose content appeared to be separate from polyuronide solubilization. In the rin mutant, the postharvest loss of these neutral sugars occurred in the absence of polygalacturonase and polyuronide solubilization. The enzyme(s) responsible for the removal of galactose and arabinose was not identified; a tomato cell wall polysaccharide containing galactose and arabinose (6:1) was not hydrolyzed by tomato β-galactosidase.
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Selected References
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- Jones T. M., Albersheim P. A gas chromatographic method for the determination of aldose and uronic Acid constituents of plant cell wall polysaccharides. Plant Physiol. 1972 Jun;49(6):926–936. doi: 10.1104/pp.49.6.926. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Labavitch J. M., Freeman L. E., Albersheim P. Structure of plant cell walls. Purification and characterization of a beta-1,4-galactanase which degrades a structural component of the primary cell walls of dicots. J Biol Chem. 1976 Oct 10;251(19):5904–5910. [PubMed] [Google Scholar]
- Labavitch J. M., Ray P. M. Turnover of cell wall polysaccharides in elongating pea stem segments. Plant Physiol. 1974 May;53(5):669–673. doi: 10.1104/pp.53.5.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Updegraff D. M. Semimicro determination of cellulose in biological materials. Anal Biochem. 1969 Dec;32(3):420–424. doi: 10.1016/s0003-2697(69)80009-6. [DOI] [PubMed] [Google Scholar]
- Wallner S. J., Bloom H. L. Characteristics of tomato cell wall degradation in vitro: implications for the study of fruit-softening enzymes. Plant Physiol. 1977 Aug;60(2):207–210. doi: 10.1104/pp.60.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wallner S. J., Walker J. E. Glycosidases in Cell Wall-degrading Extracts of Ripening Tomato Fruits. Plant Physiol. 1975 Jan;55(1):94–98. doi: 10.1104/pp.55.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]