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. 1979 Feb;63(2):298–300. doi: 10.1104/pp.63.2.298

Aspects of 8-[14C]Benzylaminopurine Metabolism in Phaseolus vulgaris1

Angelo Ramina a
PMCID: PMC542818  PMID: 16660717

Abstract

Phaseolus vulgaris L. plants were supplied through the root with [8-14C]benzylaminopurine ([14C]BA). Collections of root, apex, and leaves were made 8 and 48 hours after labeling; ethanolic extracts of tissues were purified and subjected to thin layer chromatography on silica gel and/or cellulose powder.

From [14C]BA at least two different metabolites originate, the BA riboside and ribotide. The balance among [14C]BA, the riboside, and the ribotide changed in time. The [14C]BA riboside was detected as the only labeled compound in the xylem sap collected from the stem. The biological function of BA riboside and ribotide is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong D. J., Murai N., Taller B. J., Skoog F. Incorporation of cytokinin N-benzyladenine into tobacco callus transfer ribonucleic Acid and ribosomal ribonucleic Acid preparations. Plant Physiol. 1976 Jan;57(1):15–22. doi: 10.1104/pp.57.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chen C. M., Eckert R. L. Phosphorylation of cytokinin by adenosine kinase from wheat germ. Plant Physiol. 1977 Mar;59(3):443–447. doi: 10.1104/pp.59.3.443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Divekar A. Y., Slocum H. K., Hakala M. T. N6-(delta2-isopentenyl)adenosine 5'-monophosphate: formation and effect on purine metabolism in cellular and enzymatic systems. Mol Pharmacol. 1974 May;10(3):529–543. [PubMed] [Google Scholar]
  4. Fox J. E., Chen C. Characterization of labeled ribonucleic acid from tissue grown on 14C-containing cytokinins. J Biol Chem. 1967 Oct 10;242(19):4490–4494. [PubMed] [Google Scholar]
  5. Ramina A., Pimpini F., Boniolo A., Bergamasco F. 8-[C]Benzylaminopurine Translocation in Phaseolus vulgaris. Plant Physiol. 1979 Feb;63(2):294–297. doi: 10.1104/pp.63.2.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Scholtissek C. Studies on the uptake of nucleic acid precursors into cells in tissue culture. Biochim Biophys Acta. 1968 Jun 24;158(3):435–447. doi: 10.1016/0304-4165(68)90297-3. [DOI] [PubMed] [Google Scholar]
  7. Short K. C., Torrey J. G. Cytokinins in seedling roots of pea. Plant Physiol. 1972 Feb;49(2):155–160. doi: 10.1104/pp.49.2.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Syõno K., Torrey J. G. Identification of Cytokinins of Root Nodules of the Garden Pea, Pisum sativum L. Plant Physiol. 1976 Apr;57(4):602–606. doi: 10.1104/pp.57.4.602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Walker G. C., Leonard N. J. The mode of incorporation of 6-benzylaminopurine into tobacco callus transfer ribonucleic Acid: a double labeling determination. Plant Physiol. 1974 Nov;54(5):737–743. doi: 10.1104/pp.54.5.737. [DOI] [PMC free article] [PubMed] [Google Scholar]

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