Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1979 Mar;63(3):548–551. doi: 10.1104/pp.63.3.548

Effects of Light and 3-(3,4-Dichlorophenyl)-1,1-Dimethylurea on Levels of ATP in Lemna paucicostata 6746 and a Photosynthetic Mutant with Abnormal Flowering Responses 1

Richard A Gower a, Herbert B Posner a
PMCID: PMC542867  PMID: 16660764

Abstract

The effects of light, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), and ammonium ion on pool sizes of ATP were studied in Lemna paucicostata 6746 (wild type) and a photosynthetic mutant (strain 1073) with abnormal flowering responses. Wild type fronds were capable of endogenous and phenazine methosulfate-catalyzed cyclic photophosphorylation. The endogenous cyclic photophosphorylation was inhibited by DCMU. The mutant fronds showed little endogenous but appreciable rates of phenazine methosulfate-catalyzed cyclic photophosphorylation. Treatment with DCMU during prolonged exposure to light did not result in elevated levels of ATP. Ammonium ion in the medium did not inhibit light-induced increases in pool sizes of ATP. It is concluded that the previously reported effects on flowering of DCMU, the photosynthetic mutation or ammonium ion, were not due to altered pool sizes of ATP.

Full text

PDF
548

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arnon D. I., Chain R. K. Regulation of ferredoxin-catalyzed photosynthetic phosphorylations. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4961–4965. doi: 10.1073/pnas.72.12.4961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hillman W. S., Posner H. B. Ammonium Ion and the Flowering of Lemna perpusilla. Plant Physiol. 1971 Apr;47(4):586–587. doi: 10.1104/pp.47.4.586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Malkin R., Posner H. B. On the site of function of the Rieske iron-sulfur center in the chloroplast electron transport chain. Biochim Biophys Acta. 1978 Mar 13;501(3):552–554. doi: 10.1016/0005-2728(78)90121-4. [DOI] [PubMed] [Google Scholar]
  5. Nobel P. S., Chang D. T., Wang C. T., Smith S. S., Barcus D. E. Initial ATP Formation, NADP Reduction, CO(2) Fixation, and Chloroplast Flattening Upon Illuminating Pea Leaves. Plant Physiol. 1969 May;44(5):655–661. doi: 10.1104/pp.44.5.655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. RIESKE J. S., HANSEN R. E., ZAUGG W. S. STUDIES ON THE ELECTRON TRANSFER SYSTEM. 58. PROPERTIES OF A NEW OXIDATION-REDUCTION COMPONENT OF THE RESPIRATORY CHAIN AS STUDIED BY ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPY. J Biol Chem. 1964 Sep;239:3017–3022. [PubMed] [Google Scholar]
  7. Shahak Y., Posner H. B., Avron M. Evidence for a Block between Plastoquinone and Cytochrome f in a Photosynthetic Mutant of Lemna with Abnormal Flowering Behavior. Plant Physiol. 1976 Apr;57(4):577–579. doi: 10.1104/pp.57.4.577. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES