Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1979 Mar;63(3):567–572. doi: 10.1104/pp.63.3.567

Changes in Pool Sizes of Free Amino Acids and Amides in Leaves and Plastids of Zea mays during Leaf Development 1

David J Chapman a,2, Rachel M Leech a
PMCID: PMC542871  PMID: 16660768

Abstract

The concentrations of free amino acids and amides within isolated maize (Zea mays L.) plastids were determined and compared with concentrations in the leaf tissue. The concentrations were different for each individual amino acid and varied between 1 and 10 millimolar. At five different developmental stages concentrations in the plastids were greater than those in the intact leaf tissue. During development, from the proplastid stage to the mature chloroplast, the amount of each amino acid per plastid remained relatively constant, but there were decreases in concentrations of plastid amino acids resulting from the developmental increase in plastid volume. In proplastids, the free amino acids were present in greater concentrations than those previously found to inhibit partially amino acid-synthesizing enzymes located in chloroplasts. In the chloroplasts, the molarities of the free amino acids were within the range known to inhibit amino acid-synthesizing enzymes.

Full text

PDF
567

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bauer A., Urquhart A. A., Joy K. W. Amino Acid metabolism of pea leaves: diurnal changes and amino Acid synthesis from N-nitrate. Plant Physiol. 1977 May;59(5):915–919. doi: 10.1104/pp.59.5.915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bryan J. K., Lissik E. A., Matthews B. F. Changes in Enzyme Regulation during Growth of Maize: III. Intracellular Localization of Homoserine Dehydrogenase in Chloroplasts. Plant Physiol. 1977 Apr;59(4):673–679. doi: 10.1104/pp.59.4.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hall D. O. Nomenclature for isolated chloroplasts. Nat New Biol. 1972 Jan 26;235(56):125–126. doi: 10.1038/newbio235125a0. [DOI] [PubMed] [Google Scholar]
  5. Heldt H. W., Rapley L. Specific transport of inorganic phosphate, 3-phosphoglycerate and dihydroxyacetonephosphate, and of dicarboxylates across the inner membrane of spinach chloroplasts. FEBS Lett. 1970 Oct 5;10(3):143–148. doi: 10.1016/0014-5793(70)80438-0. [DOI] [PubMed] [Google Scholar]
  6. Kirk P. R., Leech R. M. Amino Acid Biosynthesis by Isolated Chloroplasts during Photosynthesis. Plant Physiol. 1972 Aug;50(2):228–234. doi: 10.1104/pp.50.2.228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Leech R. M., Rumsby M. G., Thomson W. W. Plastid differentiation, acyl lipid, and Fatty Acid changes in developing green maize leaves. Plant Physiol. 1973 Sep;52(3):240–245. doi: 10.1104/pp.52.3.240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Leese B. M., Leech R. M. Sequential changes in the lipids of developing proplastids isolated from green maize leaves. Plant Physiol. 1976 May;57(5):789–794. doi: 10.1104/pp.57.5.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. NOGUCHI M., TAMAKI E. Studies on nitrogen metabolism in tobacco plants. A. II. Diurnal variation in the amino acid composition of tobacco leaves. Arch Biochem Biophys. 1962 Aug;98:197–205. doi: 10.1016/0003-9861(62)90173-x. [DOI] [PubMed] [Google Scholar]
  10. O'neal D., Hew C. S., Latzko E., Gibbs M. Photosynthetic carbon metabolism of isolated corn chloroplasts. Plant Physiol. 1972 Apr;49(4):607–614. doi: 10.1104/pp.49.4.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. O'neal T. D., Joy K. W. Pea leaf glutamine synthetase: regulatory properties. Plant Physiol. 1975 Jun;55(6):968–974. doi: 10.1104/pp.55.6.968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Steer B. T. Diurnal variations in photosynthetic products and nitrogen metabolism in expanding leaves. Plant Physiol. 1973 Apr;51(4):744–748. doi: 10.1104/pp.51.4.744. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES