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. 1979 Apr;63(4):687–691. doi: 10.1104/pp.63.4.687

ATP Citrate Lyase from Germinating Castor Bean Endosperm

Localization and some Properties 1

Hansjörg Fritsch a,2,3, Harry Beevers a
PMCID: PMC542897  PMID: 16660792

Abstract

ATP citrate lyase (EC 4.1.3.8) has been found in crude extracts from endosperm tissue of germinating castor bean and shows its maximum activity in 4- to 5-day-old seedlings. A strict requirement for coenzyme A and adenosine 5′-triphosphate was demonstrated. The pH optimum for the reaction is around 7.5. The unstable enzyme can be stabilized by freezing and addition of citrate and glycerol. (−)-Hydroxycitrate is a potent inhibitor. The molecular weight is about 400,000. The adenosine 5′-triphosphate citrate lyase is localized in the plastids, where it possibly plays a role in providing acetyl coenzyme A for lipid biosynthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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