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. 1979 Apr;63(4):738–743. doi: 10.1104/pp.63.4.738

Intracellular Localization of Some Key Enzymes of Crassulacean Acid Metabolism in Sedum praealtum1

Martin H Spalding a, Mark R Schmitt a, S B Ku a, Gerald E Edwards a
PMCID: PMC542908  PMID: 16660803

Abstract

The intracellular locations of six key enzymes of Crassulacean acid metabolism were determined using enzymically isolated mesophyll protoplasts of Sedum praealtum D.C. Data from isopycnic sucrose density gradient centrifugation established the chloroplastic location of pyruvate Pi dikinase, the mitochondrial location of NAD-linked malic enzyme, and exclusively nonparticulate (not associated with chloroplasts, peroxisomes, or mitochondria) locations of phosphoenolpyruvate carboxylase, NADP-linked malic enzyme, enolase, and phosphoglycerate mutase. The consequences of this enzyme distribution with respect to compartmentalization of the pathway and the transport of metabolites in Crassulacean acid metabolism are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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