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. 1979 Apr;63(4):788–791. doi: 10.1104/pp.63.4.788

Dark Carbon Dioxide Fixation under Aerobic and Anaerobic Conditions in Maize Leaves after Preillumination in the Absence of Oxygen

Ribulose 1,5-Bisphosphate Can Serve as a Primary Acceptor of Carbon Dioxide

Elisabeth Creach 1
PMCID: PMC542918  PMID: 16660813

Abstract

When dark 14CO2 fixation in maize leaves was carried out under anaerobic conditions after preillumination in the absence of O2, the 14C incorporation in aspartic acid was transient; its maximum level was very low compared with that of malic acid. The addition of 5% O2 during the dark fixation period increased the total uptake of 14CO2 and the 14C incorporation into aspartic acid.

A study of the intramolecular distribution of radioactivity showed that 71 to 76% of the 14C was located in the C4 (β-carboxyl) of malate and aspartate and the remainder in the C1. This intramolecular labeling pattern did not change during the 5- to 60-second dark 14CO2 fixation period and was scarcely altered by the presence of O2. Three degradation techniques led to similar data.

The significance of these results is discussed taking into account the known possible carboxylation pathways. It is concluded that ribulose 1,5-bisphosphate can be a primary acceptor of CO2 when maize leaves are preilluminated in the absence of O2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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