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. 1990 Jul;87(14):5233–5237. doi: 10.1073/pnas.87.14.5233

Complete amino acid sequence of bovine glia maturation factor beta.

R Lim 1, A Zaheer 1, W S Lane 1
PMCID: PMC54297  PMID: 2196564

Abstract

The protein glia maturation factor beta, isolated from bovine brain, has been sequenced by automated Edman degradation and tandem mass spectrometry of overlapped peptide fragments generated by cyanogen bromide cleavage and enzymatic digestion with trypsin, chymotrypsin, and endoproteinases Asp-N and Lys-C. The protein has 141 amino acid residues and possesses no potential N-glycosylation sites. It contains three cysteines (at positions 7, 86, and 95), three methionines (at positions 33, 101, and 102), and one tryptophan (at position 132). The blocked amino terminus as determined by tandem mass spectrometry is an N-acetylated serine. The carboxyl terminus is a histidine. To our knowledge, the sequence shows no significant homology with other sequenced proteins. The molecular weight calculated from the sequence information is 16,582.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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