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. 1979 Jun;63(6):1068–1071. doi: 10.1104/pp.63.6.1068

Malate Synthase Activity in Cotton and Other Ungerminated Oilseeds

A Survey 1

Jan A Miernyk a, Richard N Trelease a, John S Choinski Jr a,2
PMCID: PMC542971  PMID: 16660858

Abstract

Extracts from several species and varieties of ungerminated cotton seeds plus homogenates from 18 other oilseeds (representing 11 different families) were examined for malate synthase and isocitrate lyase activity. Malate synthase activities in the various cotton seeds ranged from 35 to 129% of the units per dry seed weight found in Deltapine 16 cotton. For other oilseeds, the range was from 0.3 to 58% of Deltapine 16 cotton. Castor bean (Ricinus communis L.) had the least activity per mg dry weight (12-fold lower than the next lowest species), while Pima cotton (Gossypium barbadense L.) had the highest level (8.53 units). On a per seed basis, these values were 15 and 747 nanomoles per minute.

Malate synthase activity was measurable in all seed types examined, whereas isocitrate lyase could not be detected in any of the seeds. We suggest that synthesis of malate synthase during seed development is universal among oilseeds in the absence of glyoxylate-cycle-associated isocitrate lyase activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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