Fig. 2.

(A) Flow cytometric analysis of labeling efficiency of rabbit MSCs, exemplarily for dye concentration of 10 μg/ml DiR. Light grey = unlabeled control cells, black = DiR labeled MSCs. Histogram overlay shows fluorescence intensity of the DiR signal measured on the APC-Cy7 channel (log scale) on the X-axis. The fluorescence intensity is an arbitrary unit (a.u.). The Y-axis shows the number of cells normalized to mode (percent of max; scaling each curve to 100%). (B, C) Fluorescence microscopy of rabbit MSC labeled with 10 μg DiR/ml (B: 10× magnification; C: 40× magnification). Nuclear counterstaining was performed with DAPI (blue staining).