Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1979 Jul;64(1):65–68. doi: 10.1104/pp.64.1.65

Inhibition of Soybean Cell Growth by the Adsorption of Rhizobium japonicum

Takashi Ozawa 1, Masuro Yamaguchi 1
PMCID: PMC543025  PMID: 16660916

Abstract

Soybean cells in suspension culture were inhibited in their growth by mixed culture with Rhizobium japonicum 5033. Rhizobium cells had the ability to adsorb on the surface of soybean cells. Cell envelope prepared from Rhizobium by sonic oscillation inhibited the growth of soybean cells. The growth-inhibiting activity of the cell envelope was depressed by β-glucosidase, KIO4, urea, sodium cholate, and Triton X-100, but was stable on heating at 120 C for 15 minutes. Adsorption of the cell envelope on soybean cells was depressed by only β-glucosidase. The sodium cholate-soluble fraction of the cell envelope had the growth-inhibiting activity. Results in this paper suggest that components of the Rhizobium cell surface cause the inhibition of soybean cell growth after the adsorption of the Rhizobium cell to the soybean cell.

Full text

PDF
65

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Child J. J., Larue T. A. A simple technique for the establishment of nitrogenase in soybean callus culture. Plant Physiol. 1974 Jan;53(1):88–90. doi: 10.1104/pp.53.1.88. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dever J. E., Bandurski R. S., Kivilaan A. Partial chemical characterization of corn root cell walls. Plant Physiol. 1968 Jan;43(1):50–56. doi: 10.1104/pp.43.1.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968 Apr;50(1):151–158. doi: 10.1016/0014-4827(68)90403-5. [DOI] [PubMed] [Google Scholar]
  4. Holsten R. D., Burns R. C., Hardy R. W., Hebert R. R. Establishment of symbiosis between Rhizobium and plant cells in vitro. Nature. 1971 Jul 16;232(5307):173–176. doi: 10.1038/232173a0. [DOI] [PubMed] [Google Scholar]
  5. Martz E., Steinberg M. S. The role of cell-cell contact in "contact" inhibition of cell division: a review and new evidence. J Cell Physiol. 1972 Apr;79(2):189–210. doi: 10.1002/jcp.1040790205. [DOI] [PubMed] [Google Scholar]
  6. Phillips D. A. Factors affecting the reduction of acetylene by Rhizobium-soybean cell associations in vitro. Plant Physiol. 1974 Jan;53(1):67–72. doi: 10.1104/pp.53.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Salton M. R. Structure and function of bacterial cell membranes. Annu Rev Microbiol. 1967;21:417–442. doi: 10.1146/annurev.mi.21.100167.002221. [DOI] [PubMed] [Google Scholar]
  8. Schnaitman C. A. Effect of ethylenediaminetetraacetic acid, Triton X-100, and lysozyme on the morphology and chemical composition of isolate cell walls of Escherichia coli. J Bacteriol. 1971 Oct;108(1):553–563. doi: 10.1128/jb.108.1.553-563.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES