Abstract
Using quantitative histochemical techniques, the carbohydrate levels of guard cells from open and closed stomatal apparatus of Vicia faba L. were compared. To minimize experimental error, all comparisons were between leaflets of the same pair. Stomata on one leaflet were caused to open by light and reduced CO2. The other leaflet, which was in darkness, had closed stomata. In one experiment, data were also collected on palisade parenchyma, spongy parenchyma, and epidermal cells.
Guard cell starch concentration was higher in the leaflets with closed stomata than in open stomata by 72 ± 16 millimoles per kilogram dry weight (anhydroglucosyl equivalents) (N = 117, P < 0.02). Variation in guard cell starch concentration from one part of a leaflet to another was small. The data are consistent with the hypothesis that starch degradation provides the carbon skeletons for anion synthesis in guard cells during stomatal opening.
Sucrose concentration was higher in guard cells when stomata were open than when they were closed in all three experiments (average difference = 45 ± 7 millimoles per kilogram dry weight [N = 59, P < 0.01]). The variability of sucrose concentration within test leaflets prevented an unequivocal interpretation of these results. When all data are considered, it appears that soluble sugars increase in guard cells when stomata of Vicia faba open.
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Selected References
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- Humble G. D., Raschke K. Stomatal opening quantitatively related to potassium transport: evidence from electron probe analysis. Plant Physiol. 1971 Oct;48(4):447–453. doi: 10.1104/pp.48.4.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones M. G., Outlaw W. H., Lowry O. H. Enzymic assay of 10 to 10 moles of sucrose in plant tissues. Plant Physiol. 1977 Sep;60(3):379–383. doi: 10.1104/pp.60.3.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lust W. D., Passonneau J. V., Crites S. K. The measurement of glycogen in tissues by amylo-alpha-1,4-alpha-1,6-glucosidase after the destruction of preexisting glucose. Anal Biochem. 1975 Sep;68(1):328–331. doi: 10.1016/0003-2697(75)90712-5. [DOI] [PubMed] [Google Scholar]
- Marshall J. J., Whelan W. J. Incomplete conversion of glycogen and starch by crystalline amyloglucosidase and its importance in the determination of amylaceous polymers. FEBS Lett. 1970 Jul 29;9(2):85–88. doi: 10.1016/0014-5793(70)80319-2. [DOI] [PubMed] [Google Scholar]
- Outlaw W. H., Fisher D. B., Christy A. L. Compartmentation in Vicia faba Leaves: II. Kinetics of C-Sucrose Redistribution among Individual Tissues following Pulse Labeling. Plant Physiol. 1975 Apr;55(4):704–711. doi: 10.1104/pp.55.4.704. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Outlaw W. H., Fisher D. B. Compartmentation in Vicia faba Leaves: I. Kinetics of C in the Tissues following Pulse Labeling. Plant Physiol. 1975 Apr;55(4):699–703. doi: 10.1104/pp.55.4.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Outlaw W. H., Kennedy J. Enzymic and substrate basis for the anaplerotic step in guard cells. Plant Physiol. 1978 Oct;62(4):648–652. doi: 10.1104/pp.62.4.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Outlaw W. H., Lowry O. H. Organic acid and potassium accumulation in guard cells during stomatal opening. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4434–4438. doi: 10.1073/pnas.74.10.4434. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pallas J. E., Wright B. G. Organic Acid Changes in the Epidermis of Vicia faba and Their Implication in Stomatal Movement. Plant Physiol. 1973 Mar;51(3):588–590. doi: 10.1104/pp.51.3.588. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Kirk C. A., Raschke K. Release of Malate from Epidermal Strips during Stomatal Closure. Plant Physiol. 1978 Mar;61(3):474–475. doi: 10.1104/pp.61.3.474. [DOI] [PMC free article] [PubMed] [Google Scholar]