Abstract
Synchronization of somatic embryogenesis was achieved in a carrot (Daucus carota L. cv. “Kurodagosun”) suspension culture by sieving the initial heterogeneous cell population, by density gradient centrifugation in Ficoll solutions, and by subsequent repeated centrifugations at a low speed (50g) for a short time (5 seconds), followed by transferring the cell clusters obtained, which were composed of 3 to 10 cells, to a medium containing zeatin (0.1 micromolar) but no auxin. The frequency of embryo formation reached more than 90%, and synchrony of the embryogenetic process was observed at least in the early stages of the process. The system established in the present work provides a useful system for biochemical research into the mechanisms of somatic embryogenesis.
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- Ammirato P. V. Hormonal control of somatic embryo development from cultured cells of caraway: interactions of abscisic Acid, zeatin, and gibberellic Acid. Plant Physiol. 1977 Apr;59(4):579–586. doi: 10.1104/pp.59.4.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Verma D. C., Dougall D. K. Influence of carbohydrates on quantitative aspects of growth and embryo formation in wild carrot suspension cultures. Plant Physiol. 1977 Jan;59(1):81–85. doi: 10.1104/pp.59.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]