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. 1979 Aug;64(2):281–284. doi: 10.1104/pp.64.2.281

Energy-linked Adenosine Diphosphate Accumulation by Corn Mitochondria

II. Phosphate and Divalent Cation Requirement 1

Samir Abou-Khalil a, John B Hanson a
PMCID: PMC543071  PMID: 16660949

Abstract

The requirement for phosphate and Mg2+ in energy-linked [3H] ADP accumulation by corn mitochondria has been studied. Arsenate will fully substitute for phosphate; sulfate partially substitutes; acetate, bicarbonate, and pyrophosphate are ineffective. Phosphate is also taken up by the mitochondria, but the ADP/Pi ratio varies widely with experimental treatments. ADP does not exchange with endogenous labeled phosphate, although Pi/32Pi exchange occurs.

Mg2+ is also accumulated during ADP uptake. Mg2+ can be substituted with varying efficiency by other divalent cations, but not monovalent cations. Effective cations typically increase phosphate uptake, particularly Ca2+ · Ca2+ -activated ADP accumulation is insensitive to carboxyatractyloside over a wide range of Ca2+ concentrations. When Ca2+ is substituted for Mg2+ it is not necessary to block ATP formation to secure high levels of ADP accumulation, since Ca2+ will divert energy from ATP formation into ion uptake.

It is suggested that the transport mechanism may carry out a concerted transport of ADP and phosphate with bound divalent cation. The phosphate transporter may be involved, or alternatively a special mechanism for trivalent anion transport may exist which acts cooperatively with the phosphate transporter.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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