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. 1990 Jul;87(14):5307–5311. doi: 10.1073/pnas.87.14.5307

Purification of a DNA supercoiling factor from the posterior silk gland of Bombyx mori.

T Ohta 1, S Hirose 1
PMCID: PMC54312  PMID: 2164676

Abstract

A protein factor with an estimated molecular mass of 50 kDa has been purified to homogeneity from the silk gland of Bombyx mori. In the presence of a molar excess of this factor and eukaryotic DNA topoisomerase II, relaxed circular DNA is converted to the negatively supercoiled form. Eukaryotic DNA topoisomerase I cannot substitute for eukaryotic DNA topoisomerase II in the supercoiling reaction. The reaction is dependent on ATP and is inhibited by VP-16, a specific inhibitor of eukaryotic DNA topoisomerase II. When DNA topoisomerase I is subsequently added to the supercoiling reaction mixture, the supercoiled DNA becomes relaxed. These results suggest that when both the 50-kDa protein and eukaryotic DNA topoisomerase II are present in excess, unconstrained negative supercoils are introduced into DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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