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. 1979 Oct;64(4):594–599. doi: 10.1104/pp.64.4.594

A Cytokinin-binding Protein from Wheat Germ

Isolation by Affinity Chromatography and Properties 1,2

F Hardy Moore III a,3
PMCID: PMC543144  PMID: 16661016

Abstract

A cytokinin-binding protein has been isolated from wheat germ via ammonium sulfate precipitation, carboxymethyl Sephadex chromatography, and affinity chromatography on a column substituted with a derivative of kinetin riboside. On Sephadex G-200, the protein migrated with an apparent molecular weight of 122,000 daltons. The dissociation constant for kinetin was determined by equilibrium dialysis to be 1.2 micromolar; N6-benzylaminopurine and N6-(Δ2-isopentenyl)adenine were also strongly bound. Little affinity was exhibited toward either cis-zeatin or trans-zeatin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berridge M. V., Ralph R. K., Letham D. S. The binding of kinetin to plant ribosomes. Biochem J. 1970 Aug;119(1):75–84. doi: 10.1042/bj1190075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berridge M. V., Ralph R. K. Some effects of kinetin on floated Chinese cabbage leaf discs. Biochim Biophys Acta. 1969 May 20;182(1):266–269. doi: 10.1016/0005-2787(69)90547-4. [DOI] [PubMed] [Google Scholar]
  3. Fox J. E., Erion J. L. A cytokinin binding protein from higher plant ribosomes. Biochem Biophys Res Commun. 1975 May 19;64(2):694–700. doi: 10.1016/0006-291x(75)90376-9. [DOI] [PubMed] [Google Scholar]
  4. Humayun M. Z., Jacob T. M. Immunologic studies on nucleic acids and their components. II. Reversible inhibition of anti-nucleoside antibodies in aqueous pyridine and its application in antibody purification. Biochim Biophys Acta. 1974 Apr 27;349(1):84–95. doi: 10.1016/0005-2787(74)90011-2. [DOI] [PubMed] [Google Scholar]
  5. LETHAM D. S. ZEATIN, A FACTOR INDUCING CELL DIVISION ISOLATED FROM ZEA MAYS. Life Sci. 1963 Aug;8:569–573. doi: 10.1016/0024-3205(63)90108-5. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Miller C. O. Evidence for the natural occurrence of zeatin and derivatives: compounds from maize which promote cell division. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1052–1058. doi: 10.1073/pnas.54.4.1052. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mornet R., Theiler J. B., Leonard N. J. Active cytokinins: photoaffinity labeling agents to detect binding. Plant Physiol. 1979 Oct;64(4):600–610. doi: 10.1104/pp.64.4.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nimmo I. A., Bauermeister A. A theoretical analysis of the use of zonal gel filtration in the detection and purification of protein-ligand complexes. Biochem J. 1978 Feb 1;169(2):437–440. doi: 10.1042/bj1690437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Polya G. M., Bowman J. A. Ligand Specificity of a High Affinity Cytokinin-binding Protein. Plant Physiol. 1979 Sep;64(3):387–392. doi: 10.1104/pp.64.3.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Strange R. C., Nimmo I. A., Percy-Robb I. W. Binding of bile acids by 100 000g supernatants from rat liver. Biochem J. 1977 Mar 15;162(3):659–664. doi: 10.1042/bj1620659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Takegami T., Yoshida K. Isolation and purification of cytokinin binding protein from tobacco leaves by affinity column chromatography. Biochem Biophys Res Commun. 1975 Nov 17;67(2):782–789. doi: 10.1016/0006-291x(75)90881-5. [DOI] [PubMed] [Google Scholar]
  14. Yoshida K., Takegami T. Isolation of cytokinin binding protein from tobacco leaves by bioaffinity chromatography and its partial characterization. J Biochem. 1977 Mar;81(3):791–799. doi: 10.1093/oxfordjournals.jbchem.a131517. [DOI] [PubMed] [Google Scholar]

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