Abstract
Using the inhibitor of RNA synthesis, 4,5′,8-trimethylpsoralen plus near ultraviolet light, the half-life of the rate-limiting RNA species required for the induction of nitrate reductase in XD cells of tobacco was estimated to be 4 hours. Amino acids, the end product of nitrate assimilation, reduced the half-life to 1 hour. It is suggested that amino acids exert a posttranscriptional control on the enzyme level.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ferrari T. E., Yoder O. C., Filner P. Anaerobic nitrite production by plant cells and tissues: evidence for two nitrate pools. Plant Physiol. 1973 Mar;51(3):423–431. doi: 10.1104/pp.51.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Filner P. Regulation of nitrate reductase in cultured tobacco cells. Biochim Biophys Acta. 1966 May 5;118(2):299–310. doi: 10.1016/s0926-6593(66)80038-3. [DOI] [PubMed] [Google Scholar]
- Heimer Y. M., Ben-Hur E., Riklis E. Photosensitized inhibition of nitrate reductase induction by 4,5',8-trimethylpsoralen and near ultraviolet light. Biochim Biophys Acta. 1978 Jul 24;519(2):499–506. doi: 10.1016/0005-2787(78)90102-8. [DOI] [PubMed] [Google Scholar]
- Heimer Y. M., Ben-Hur E., Riklis E. Psoralen and near ultraviolet light; a probe for study of control of protein synthesis. Nature. 1977 Jul 14;268(5616):170–171. doi: 10.1038/268170a0. [DOI] [PubMed] [Google Scholar]
- Heimer Y. M., Filner P. Regulation of the nitrate assimilation pathway in cultured tobacco cells. 3. The nitrate uptake system. Biochim Biophys Acta. 1971 Feb 23;230(2):362–372. doi: 10.1016/0304-4165(71)90223-6. [DOI] [PubMed] [Google Scholar]
- Heimer Y. M., Wray J. L., Filner P. The effect of tungstate on nitrate assimilation in higher plant tissues. Plant Physiol. 1969 Aug;44(8):1197–1199. doi: 10.1104/pp.44.8.1197. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zielke H. R., Filner P. Synthesis and turnover of nitrate reductase induced by nitrate in cultured tobacco cells. J Biol Chem. 1971 Mar 25;246(6):1772–1779. [PubMed] [Google Scholar]