Abstract
Polysome profiles were examined from lyophilized peel tissue of ripening pear (Pyrus communis, L. var. Passe-Crassane). Messenger RNA chains bearing up to eight ribosomes (octamers) were resolved and exhibited the highest absorption peak when ribonuclease activity was eliminated during extraction. Neither normal ripening nor the increase of large polyribosomes that normally accompanies ripening and senescence of the fruit occurred when pretreatment at 0 C was omitted. Normal ripening and increase of large polyribosomes would, however, be initiated by an ethylene treatment. The size distribution of the polyribosomes remained essentially constant throughout a 4-month cold storage; there was, however, a large increase in ribosomes by the 12th week of storage.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Breen M. D., Whitehead E. I., Kenefick D. G. Requirement for extraction of polyribosomes from barley tissue. Plant Physiol. 1972 May;49(5):733–739. doi: 10.1104/pp.49.5.733. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davies E., Larkins B. A., Knight R. H. Polyribosomes from peas: an improved method for their isolation in the absence of ribonuclease inhibitors. Plant Physiol. 1972 Nov;50(5):581–584. doi: 10.1104/pp.50.5.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davies E., Larkins B. A. Polyribosomes from Peas: II. Polyribosome Metabolism during Normal and Hormone-induced Growth. Plant Physiol. 1973 Oct;52(4):339–345. doi: 10.1104/pp.52.4.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartmann C. J., Drouet A. G. Pear malic enzyme: some physical and immunochemical properties. Rev Can Biol. 1979 Jun;38(2):101–104. [PubMed] [Google Scholar]
- Ku L. L., Romani R. J. The ribosomes of pear fruit. Their synthesis during the climacteric and the age-related compensatory response to ionizing radiation. Plant Physiol. 1970 Apr;45(4):401–407. doi: 10.1104/pp.45.4.401. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ramagopal S., Hsiao T. C. Polyribosomes from maize leaves. Isolation at high pH and amino acid incorporation. Biochim Biophys Acta. 1973 Mar 28;299(3):460–467. doi: 10.1016/0005-2787(73)90270-0. [DOI] [PubMed] [Google Scholar]
- Romani R., French K. Temperature-dependent Changes in the Polysomal Population of Senescent (Ripening) Pear Fruit. Plant Physiol. 1977 Dec;60(6):930–932. doi: 10.1104/pp.60.6.930. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STAEHELIN T., WETTSTEIN F. O., OURA H., NOLL H. DETERMINATION OF THE CODING RATIO BASED ON MOLECULAR WEIGHT OF MESSENGER RIBONUCLEIC ACID ASSOCIATED WITH ERGOSOMES OF DIFFERENT AGGREGATE SIZE. Nature. 1964 Jan 18;201:264–270. doi: 10.1038/201264a0. [DOI] [PubMed] [Google Scholar]
- Stone A. B. A simplified method for preparing sucrose gradients. Biochem J. 1974 Jan;137(1):117–118. doi: 10.1042/bj1370117. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vassart G., Dumont J. E., Cantraine F. R. Translational control of protein synthesis: a simulation study. Biochim Biophys Acta. 1971 Oct;247(3):471–485. doi: 10.1016/0005-2787(71)90034-7. [DOI] [PubMed] [Google Scholar]