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. 1974 Mar;53(3):352–359. doi: 10.1104/pp.53.3.352

Phytochrome Stability in Vitro

I. Effect of Metal Ions 1

Stephen G Lisansky a,2,3, Arthur W Galston a
PMCID: PMC543222  PMID: 16658705

Abstract

Photoreversible phytochrome disappears from etiolated tissue upon actinic irradiation. Such disappearance, of possible physiological importance, involves several processes, at least one of which is accelerated by metals in vivo. Purified phytochrome from oat (Avena sativa L. cv. Garry) coleoptiles is greatly stabilized in vitro by scrupulous removal of metal impurities via chelating agents. Such stabilized phytochrome decays rapidly upon the addition of about 10 μm Hg2+, Cd2+, Cu2+, and Zn2+, all of which probably act on sulfhydryl groups. Other tested metals and growth factors were much less active or inactive. The metals effective in promoting decay do not affect the Pfr → Pr reversion process. This supports other evidence indicating the possible physiological importance of phytochrome “decay.”

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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