Figure 6. U2OS tumor growth in SCID mice is inhibited after expressing Ppm1e shRNA or miR-135b-5p.

Stable U2OS cells, with Ppm1e shRNA (“-1”), Vec-miR-135b (“L1”) or empty vector (“Vec”), were inoculated into the SCID mice via s.c. injection. When the tumors were around 100 mm³ in volume, the recording were started. Tumor volume (A) and mice body weight (D), which subtracting estimated tumor weight) were recorded every week for a total of 6 weeks (Week1 to Week7); Estimated daily tumor growth, in mm3 per day, was also calculated (B); At week-7, tumors of each group were isolated and weighted (C); Expressions of Ppm1e mRNA (E), miR-135b-5 (F) in tumor tissue lysates were also tested. For each group, n = 10. *p < 0.05 vs. “Vec” control tumors.