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. 2017 Mar 3;8(16):26927–26940. doi: 10.18632/oncotarget.15879

Figure 4. Triptolide and CD44 siRNA reduced the proliferation and self-renewal of putative lung cancer stem cells expressing CD44.

Figure 4

(A, C) Effects of triptolide (A) or CD44 siRNA (C) on the number of primary-, secondary and tertiary-pulmospheres generated from A549 cells. Cells were treated with triptolide (12.5, 25 and 50 nM) or CD44 siRNA and the frequency of pulmospheres determined as described in the Materials and Methods section. (B, D) Images of primary, secondary and tertiary pulmospheres generated from triptolide (B)- or CD44 siRNA (D)- treated A549 cells. At least three independent assays were carried for these assays. *P < 0.05.