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. 1974 Mar;53(3):491–495. doi: 10.1104/pp.53.3.491

Photosynthesis in the Higher Plant, Vicia faba

III. Serine, a Precursor of the Tricarboxylic Acid Cycle 1

S Sherrill Kent a,2, Frederick D Pinkerton a, Gary A Strobel a
PMCID: PMC543263  PMID: 16658730

Abstract

Evidence is presented to support the hypothesis that serine, rather than 3-phosphoglycerate of the Calvin cycle, is a precursor of the tricarboxylic acid cycle during photosynthesis by the higher plant, Vicia faba. Identification of the serine intermediate is based upon a unique C1 > C2 > C3 isotope distribution for that metabolite following the fixation of 14CO2. This labeling pattern, while incompatible with an origin either in the Calvin cycle or the glycolate pathway, satisfies a critical criterion for the 3-carbon precursor of the anomalously labeled organic acids. The predominant carboxyl carbon atom labeling of serine reflects either a mixing of two pools of that metabolite, ie., C1 = C2 > C3 and C1 > C2 = C3, or a higher order of complexity in its synthesis. An anomalous C1 = C2 > C3 < C4 distribution for aspartate, however, suggests an origin by the carboxylation of a 3-carbon intermediate related to serine which has a C1 = C2 > C3 distribution. The latter distribution has been proposed for the serine intermediate of the postulated formate pathway. This pathway is described by the generalized metabolic sequence: CO2 → formate → serine → organic acids. Corresponding carbon atom distributions for citrate (C1 > C2), aspartate (C2 > C3), and serine (C2 > C3) belie a precursor-product relationship with alanine (C2 = C3), which is a molecular parameter of the Calvin cycle product, 3-phosphoglycerate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARONOFF S. Metabolism of soybean leaves. III. The organic acids produced in short-time photosynthesis. Arch Biochem Biophys. 1951 Jul;32(2):237–248. doi: 10.1016/0003-9861(51)90269-x. [DOI] [PubMed] [Google Scholar]
  2. BAILLIE L. A. Determination of liquid scientillation counting efficiency by pulse height shift. Int J Appl Radiat Isot. 1960 May;8:1–7. doi: 10.1016/0020-708x(60)90153-8. [DOI] [PubMed] [Google Scholar]
  3. BUSCH H., HURLBERT R. B., POTTER V. R. Anion exchange chromatography of acids of the citric acid cycle. J Biol Chem. 1952 May;196(2):717–727. [PubMed] [Google Scholar]
  4. Bruin W. J., Nelson E. B., Tolbert N. E. Glycolate pathway in green algae. Plant Physiol. 1970 Sep;46(3):386–391. doi: 10.1104/pp.46.3.386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burns L. C., O'Neal R. M., Koeppe R. E. Labeling patterns in glutamic acid in Nicotiana rustica L. from carbon-14 dioxide. J Am Chem Soc. 1967 Jul 19;89(15):3938–3939. doi: 10.1021/ja00991a069. [DOI] [PubMed] [Google Scholar]
  6. Consden R., Gordon A. H., Martin A. J. Qualitative analysis of proteins: a partition chromatographic method using paper. Biochem J. 1944;38(3):224–232. doi: 10.1042/bj0380224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cossins E. A., Sinha S. K. The interconversion of glycine and serine by plant tissue extracts. Biochem J. 1966 Nov;101(2):542–549. doi: 10.1042/bj1010542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gibbs M. THE POSITION OF C IN SUNFLOWER LEAF METABOLITES AFTER EXPOSURE OF LEAVES TO SHORT PERIOD PHOTOSYNTHESIS AND DARKNESS IN AN ATMOSPHERE OF CO(2). Plant Physiol. 1951 Jul;26(3):549–556. doi: 10.1104/pp.26.3.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hess J. L., Tolbert N. E. Glycolate pathway in algae. Plant Physiol. 1967 Mar;42(3):371–379. doi: 10.1104/pp.42.3.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hess J. L., Tolbert N. E. Glycolate, glycine, serine, and glycerate formation during photosynthesis by tobacco leaves. J Biol Chem. 1966 Dec 10;241(23):5705–5711. [PubMed] [Google Scholar]
  11. Kent S. S. Complete stereochemical distribution of 14 C-isotope in citrate. Anal Biochem. 1972 Oct;49(2):393–406. doi: 10.1016/0003-2697(72)90442-3. [DOI] [PubMed] [Google Scholar]
  12. Kent S. S. Distribution of 14C-isotope in stereoisomers of serine. Can J Biochem. 1973 Nov;51(11):1475–1478. doi: 10.1139/o73-195. [DOI] [PubMed] [Google Scholar]
  13. Kent S. S. Photosynthesis in the higher plant Vicia faba. I. Anomalous distributions of 14 CO 2 -isotope in citrate and glutamate. J Biol Chem. 1972 Nov 25;247(22):7288–7292. [PubMed] [Google Scholar]
  14. Kent S. S. Photosynthesis in the higher plant Vicia faba. II. The non-Calvin cycle origin of acetate and its metabolic relationship to the photosynthetic origin of formate. J Biol Chem. 1972 Nov 25;247(22):7293–7302. [PubMed] [Google Scholar]
  15. MORTIMER D. C. Anomalous results in the periodic acid oxidation of serine. Can J Biochem Physiol. 1959 Aug;37(8):919–925. [PubMed] [Google Scholar]
  16. RABSON R., TOLBERTNE, KEARNEY P. C. Formation of serine and glyceric acid by the glycolate pathway. Arch Biochem Biophys. 1962 Jul;98:154–163. doi: 10.1016/0003-9861(62)90161-3. [DOI] [PubMed] [Google Scholar]
  17. SAKAMI W., LAFAYE J. M. Formation of formate and labile methyl groups from acetone in the intact rat. J Biol Chem. 1950 Nov;187(1):369–378. [PubMed] [Google Scholar]
  18. Yamamoto Y., Beevers H. Malate Synthetase in Higher Plants. Plant Physiol. 1960 Jan;35(1):102–108. doi: 10.1104/pp.35.1.102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. ZELITCH I. THE RELATION OF GLYCOLIC ACID SYNTHESIS TO THE PRIMARY PHOTOSYNTHETIC CARBOXYLATION REACTION IN LEAVES. J Biol Chem. 1965 May;240:1869–1876. [PubMed] [Google Scholar]

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