Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1974 Mar;53(3):509–511. doi: 10.1104/pp.53.3.509

Phospholipid Degradation in Frozen Plant Cells Associated with Freezing Injury 1,2

Shizuo Yoshida a, Akira Sakai a
PMCID: PMC543271  PMID: 16658734

Abstract

A striking degradation of phosphatidylcholine into phosphatidic acid was observed in the cortical tissues of less hardy poplar (Poplus euramericani cv. gelrica), when the tissues were frozen below a lethal temperature. No change in phospholipids was detected during freezing or even after thawing in the cortical tissues of hardy poplar which survived slow freezing to −30 C or even immersion in liquid N2 after prefreezing to −50 C. The degradation of phosphatidylcholine during freezing appears to be intimately associated with freezing injury.

Full text

PDF
509

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  2. Gusta L. V., Weiser C. J. Nucleic Acid and protein changes in relation to cold acclimation and freezing injury of korean boxwood leaves. Plant Physiol. 1972 Jan;49(1):91–96. doi: 10.1104/pp.49.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Heber U. Freezing injury in relation to loss of enzyme activities and protection against freezing. Cryobiology. 1968 Nov-Dec;5(3):188–201. doi: 10.1016/s0011-2240(68)80163-4. [DOI] [PubMed] [Google Scholar]
  4. Henderson I. W., Angeloff L. Post-freeze-thaw viabilities of spleen slices by measurement of nucleic acid and protein syntheses. Cryobiology. 1969 Nov-Dec;6(3):249–256. doi: 10.1016/s0011-2240(69)80356-1. [DOI] [PubMed] [Google Scholar]
  5. KATES M. Lecithinase systems in sugar beet, spinach, cabbage, and carrot. Can J Biochem Physiol. 1954 Sep;32(5):571–583. [PubMed] [Google Scholar]
  6. Sukumaran N. P., Weiser C. J. Freezing injury in potato leaves. Plant Physiol. 1972 Nov;50(5):564–567. doi: 10.1104/pp.50.5.564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Tzur R., Shapiro B. Purification of phospholipase D from peanuts. Biochim Biophys Acta. 1972 Oct 5;280(2):290–296. doi: 10.1016/0005-2760(72)90096-3. [DOI] [PubMed] [Google Scholar]
  8. Weiser C. J. Cold Resistance and Injury in Woody Plants: Knowledge of hardy plant adaptations to freezing stress may help us to reduce winter damage. Science. 1970 Sep 25;169(3952):1269–1278. doi: 10.1126/science.169.3952.1269. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES