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Restoration of plaque size by complementation with hyperfusogenic gB. Vero cells were transfected with empty-vector DNA (V) or a plasmid encoding gB 3A or gB 876T. After 24 h, the cells were infected with gB 3A-1 or gB 3A-2 virus for 2 h and overlaid with methylcellulose. Plaques were stained, and the radii of at least 40 randomly selected plaques from each virus were measured to calculate the plaque area. Each data point represents a single plaque.
