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. 1979 Nov;64(5):702–705. doi: 10.1104/pp.64.5.702

Respiratory Changes with Chilling Injury of Soybeans

A Carl Leopold c, Mary E Musgrave c
PMCID: PMC543335  PMID: 16661038

Abstract

The leakage of solutes from cotyledons of soybeans (cv. Chippewa 64) was markedly stimulated by a chilling treatment (1 to 4 C) during the 1st minute of imbibition, but chilling after even 1 minute of water uptake resulted in little or no leakage increase. The respiratory rate of soybean particles was reduced more than 60% if a chilling treatment (15 minutes at 1 to 4 C) was given during the first minutes of imbibition, and little or no reduction was obtained if the chilling treatment was begun at 5 to 15 minutes after the start of imbibition. Using KCN as an inhibitor of cytochrome oxidase pathway of respiration and salicylhydroxamic acid as an inhibitor of the alternative pathway, it was found that the chilling injury involved a major reduction in the cytochrome pathway in whole axes and cotyledons and an engagement of the alternative pathway of respiration in cotyledon tissue. The suggestion is made that the chilling injury involves lesions resulting from temperature stress during the reorganization of membranes with water entry, and that both the leakage and the respiratory effects are consequences of these membrane lesions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bahr J. T., Bonner W. D., Jr Cyanide-insensitive respiration. I. The steady states of skunk cabbage spadix and bean hypocotyl mitochondria. J Biol Chem. 1973 May 25;248(10):3441–3445. [PubMed] [Google Scholar]
  2. Bramlage W. J., Leopold A. C., Parrish D. J. Chilling Stress to Soybeans during Imhibition. Plant Physiol. 1978 Apr;61(4):525–529. doi: 10.1104/pp.61.4.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Christiansen M. N. Periods of sensitivity to chilling in germinating cotton. Plant Physiol. 1967 Mar;42(3):431–433. doi: 10.1104/pp.42.3.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Duke S. H., Schrader L. E., Miller M. G. Low Temperature Effects on Soybean (Glycine max [L.] Merr. cv. Wells) Mitochondrial Respiration and Several Dehydrogenases during Imbibition and Germination. Plant Physiol. 1977 Nov;60(5):716–722. doi: 10.1104/pp.60.5.716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Parrish D. J., Leopold A. C. Transient changes during soybean imbibition. Plant Physiol. 1977 Jun;59(6):1111–1115. doi: 10.1104/pp.59.6.1111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pollock B. M. Imbibition temperature sensitivity of lima bean seeds controlled by initial seed moisture. Plant Physiol. 1969 Jun;44(6):907–911. doi: 10.1104/pp.44.6.907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Pollock B. M., Toole V. K. Imbibition period as the critical temperature sensitive stage in germination of lima bean seeds. Plant Physiol. 1966 Feb;41(2):221–229. doi: 10.1104/pp.41.2.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Theologis A., Laties G. G. Relative Contribution of Cytochrome-mediated and Cyanide-resistant Electron Transport in Fresh and Aged Potato Slices. Plant Physiol. 1978 Aug;62(2):232–237. doi: 10.1104/pp.62.2.232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Woodstock L. W., Pollock B. M. Physiological Predetermination: Imbibition, Respiration, and Growth of Lima Bean Seeds. Science. 1965 Nov 19;150(3699):1031–1032. doi: 10.1126/science.150.3699.1031. [DOI] [PubMed] [Google Scholar]

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