Abstract
Chromatic adaptation is the process by which blue-green algae alter the rates of biliprotein synthesis in response to changes in the color of available light. We have examined the control of phycoerythrin synthesis during the early stages of chromatic adaptation in Fremyella diplosiphon using fluorescence spectroscopy and 35S-labeling of polypeptides. Phycoerythrin synthesis begins within 45 to 90 minutes after transfer of cells from red to green light, but is blocked by rifamycin. Transfer of cells from green to red light stops phycoerythrin synthesis with a t½ = 45 minutes, as does the addition of rifamycin in green light. Transfer from green light to darkness slows but does not stop phycoerythrin synthesis. Gel electrophoresis of labeled polypeptides, both soluble and membrane-bound, shows that the synthesis of some polypeptides other than phycoerythrin are also affected by changes in light. These data suggest that chromatic adaptation involves gene regulation at the transcriptional level.
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- Bennett A., Bogorad L. Complementary chromatic adaptation in a filamentous blue-green alga. J Cell Biol. 1973 Aug;58(2):419–435. doi: 10.1083/jcb.58.2.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
- Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
- Haury J. F., Bogorad L. Action Spectra for Phycobiliprotein Synthesis in a Chromatically Adapting Cyanophyte, Fremyella diplosiphon. Plant Physiol. 1977 Dec;60(6):835–839. doi: 10.1104/pp.60.6.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Miller S. S., Bogorad L. Purification and Characterization of RNA Polymerase from Fremyella diplosiphon. Plant Physiol. 1978 Dec;62(6):995–999. doi: 10.1104/pp.62.6.995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tandeau de Marsac N. Occurrence and nature of chromatic adaptation in cyanobacteria. J Bacteriol. 1977 Apr;130(1):82–91. doi: 10.1128/jb.130.1.82-91.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamanaka G., Glazer A. N., Williams R. C. Cyanobacterial phycobilisomes. Characterization of the phycobilisomes of Synechococcus sp. 6301. J Biol Chem. 1978 Nov 25;253(22):8303–8310. [PubMed] [Google Scholar]
- de Marsac N. T., Cohen-bazire G. Molecular composition of cyanobacterial phycobilisomes. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1635–1639. doi: 10.1073/pnas.74.4.1635. [DOI] [PMC free article] [PubMed] [Google Scholar]