(
a) Cellular views of bright-field or 405 nm or 641 nm conventional imaging are presented from left to right, respectively. It is the 4th cell from
Figure 5d with red line indicating cellular outline. The boxes showed two different regions with dotted (i) or no obviously recognizable (ii) structure in 641-conventional imaging. (
b) Reconstructed STORM images of regions corresponding to yellow (i) or green (ii) box in (
a) are shown as upper row, and zoomed-in views present in lower row with localization number shown in the lower-right corner. Discontinuous clusters with extremely low number of localizations is observed in the yellow box(i) whereas continuous fine-scale nanostructure is identified in the green box (ii). STORM images are presented as rainbow color-coded z-axis information (color bar at the bottom, −350 to 350 nm). Scale bars in (
a) and (
b) are 1 μm and 300 nm, respectively.