Figure 6. Experimental activation of MS1 neurons elicits calcium responses in FRU neurons.

(A) GCaMP6m increase (△F) in FRU neurons of a dissected male brain in which MS1 neurons are activated by P2X2 expression and ATP perfusion. Antero-dorsal (left) and posterior (right) views are presented using the ‘fire’ look-up table. AL: antennal lobes. Arrows point to a pair of neurons that may be aSP4. Scale bar: 100 µm. (B) Normalized GCaMP6m response (△F/F⁰) in the cell bodies of mAL and P1 neurons, and the arch region in male (M), female (F), or negative control (C) brains. Female brains do not exhibit calcium responses in P1 neurons because these neurons are male specific. Flies carrying UAS-P2X2, fru-LexA, and LexAop-GCaMP6m, but not MS1-Gal4 served as negative controls. Fluorescence traces (top) and peak responses (bottom) are presented. Gray rectangles indicate 2.5 mM ATP perfusion. N = 4–9. Student’s t test (B).

DOI: http://dx.doi.org/10.7554/eLife.23130.014

Figure 6—figure supplement 1. MS1 stimulation induces calcium responses in P1.

P2X2 was expressed under the control of MS1-Gal4, while GCaMP6m was expressed under the control of R71G01-LexA. A posterior view is presented using the ‘fire’ look-up table. Scale bar: 100 µm.

DOI: http://dx.doi.org/10.7554/eLife.23130.015