| Gene/Traget | Forwad sequence (5′-3′) | Reverse sequence (5′-3′) |
| OCT4 | CCTCACTTCACTGCACTGTA | CAGGTTTTCTTTCCCTAGCT |
| NANOG | TGAACCTCAGCTACAAACAG | TGGTGGTAGGAAGAGTAAAG |
| DPPA2 | CCCCTCCCTTGCCAACCATT | CACTGCCTTGCGTTTCCTCGA |
| AFP | AGCTTGGTGGTGGATGAAAC | CCCTCTTCAGCAAAGCAGAC |
| CD34 | GCGCTTTGCTTGCTGAGTTT | CCAAGGGTACTAGGTATTGC |
| NCAM | ATGGAAACTCTATTAAAGTGAACCTG | TAGACCTCATACTCAGCATTCCAGT |
| ACTIN | TGAAGTGTGACGTGGACATC | GGAGGAGCAATGATCTTGAT |
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