Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun;23(6):899–909. doi: 10.1261/rna.056788.116

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Rihan et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

FIGURE 3. — The G-motif is required to target reporter mRNA in axons of differentiated NSC-34 cells. (A) Representative pictures of cells expressing the indicated constructs. FISH was performed using LacZ probes after 3 d of differentiation. Scale bar, 10 µm. Insets on the bottom of each panel are enlarged images of boxed sections. (B) Quantitative analysis of localization of reporter RNAs in axons as illustrated in A. Scatter plots show the number of mRNA spots localized in the distal segment of axons with the FL-wt (n = 19), 5′UTR + CS (n = 19), 3′UTR (n = 45), and FL-rev (n = 29) reporters (n corresponds to the number of cells that were analyzed). Mean and SEM are shown. Statistical significance was calculated using the Wilcoxon test. (***) P < 0.001.