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. 2017 Jun;23(6):910–926. doi: 10.1261/rna.060640.117

FIGURE 2.

FIGURE 2.

Knockdown of Skiv2l2 in P19 cells enhances differentiation into cardiomyocytes. (A) Western blot to detect SKIV2L2 protein in P19 whole-cell extract from cells transfected with control siRNA, Skiv2l2 siRNA, or grown in 0.1% DMSO. SKIV2L2 protein levels (118 kD) were normalized to β-actin (42 kD). (B) qRT-PCR of Skiv2l2 mRNA in P19 cells. Skiv2l2 mRNA levels in control siRNA-treated cells, 0.1% DMSO-treated cells, and Skiv2l2 siRNA-treated cells were measured via ΔCq values and normalized to β-actin (error bars represent ±SD for n = 4). (C) qRT-PCR of myocyte differentiation transcription factors in P19 cells. GATA4 and Nkx2.5 mRNA levels calculated as in B (error bars represent ±SD for n = 3). (D) qRT-PCR of myocyte differentiation markers in P19 cells. Fabp3 and Desmin mRNA levels calculated as in B (error bars represent ±SD for n = 3). (E) qRT-PCR of pluripotency markers Sox2 and Nanog in P19 cells. Sox2 and Nanog mRNA levels calculated as in B (error bars represent ±SD for n = 3).